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KMID : 1098420100180040231
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Korean Journal of Medicinal Crop Science
2010 Volume.18 No. 4 p.231 ~ p.237
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Effects of Water Extract of Glycyrrhiza uralensis on β-Hexosaminidase Release and Expression of the Cytokines of RBL-2H3 Mast Cells
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Kim Jeong-Mi
Kim Dae-Jung
Kim Tae-Hyuk
Baek Jong-Mi
Kim Hyun-Sook
Choe Myeon
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Abstract
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The present study was conducted to investigate the anti-allergic reaction with Glycyrrhiza uralensis. We examined cell viability, β-hexosaminidase release, IL-4 and IL-13 mRNA expression from RBL-2H3 cell after pre-treatment with 0, 100, 250, 500, 1000㎍/㎖ of Glycyrrhiza uralensis water extracts. Effects of Glycyrrhiza uralensis on the degranulation and pro-inflammatory cytokines (IL-4 and IL-13) expression were evaluated with β-hexosaminidase assay, and RT-PCR analysis. We observed that Glycyrrhiza uralensis concentrations from 100㎍/㎖ to 1000㎍/㎖ had no effect on cell survival. The release of β-hexosaminidase decreased significantly with all concentrations of Glycyrrhiza uralensis extracts. The expression of the IL-4 and IL-13 mRNA were decreased by Glycyrrhiza uralensis in dose-dependent manner. These results that Glycyrrhiza uralensis has an anti-histamin effects and controls IL-4, IL-13 secretion on allergic reaction.
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KEYWORD
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Glycyrrhiza uralensis, Anti-Allergic, RBL-2H3, IL-4, IL-13, β-hexosaminidase
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